Position is located within the Medical Research Service at the Veterans Affairs (VA) Medical Center in Durham, NC. Duties include but are not limited to the following:
Instructing others in the methods used within the laboratory;
Performing experiments using transgenic and knockout mice;
Performing systematic blood pressure measurements in awake mice;
Collecting urine specimens from live mice using metabolic cages;
Performing experiments using immunohistochemistry to examine cellular/molecular determinants of glomerular injury;
Performing flow cytometric analysis to analyze protein and mRNA;
Extracting RNA and analyzing changes in message in kidneys of mice;
Performing Western blot analysis of protein expression;
Recording experiments to include protocols, measurements, observations;
Retrieving and interpreting data from notebooks as requested.
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The successful candidate will examine the dynamic relationships between sulfated components of extracellular matrix, Wingless (Wnt) and TGF signaling pathways, and forkhead (Fox) transcription factors as they apply to the differentiation of alveolar epithelium in the adult lung. He or she will study the molecular biology of signaling pathways and transcription factor interactions in control of relevant transcriptive activity in normal isolated alveolar epithelial cells cultured on modified extracellular matrices. Biological outcomes of manipulated whole animals with targeted gene modifications will be studied in appropriate disease models of pulmonary fibrosis.
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Minimum requirements BS or MS degree in Cell Biology, Biochemistry, Molecular Biology, or related field with 5+ years experience…
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The QC Scientist 1 is responsible for independent problem solving and the individual is specialized in at one or more discipline area within the department. The primary responsibility is to execute components of a project, both internal method improvement projects and larger customer technology/method transfer and qualification projects with the objective of revenue, profit plan, and manufacturing schedule attainment. The QC Scientist 1 supports the project management and quality systems related to the routine testing and technology transfer. The Scientist may have a lead role in method development and transfer projects from the customer to the QC laboratory. The incumbent represents the department as a discipline or technology subject matter expert, assisting with the development, trouble-shooting, improvement, and implementation of analytical methodology into the group. The individual interacts with all levels and departments within the company, assuring procedures, protocols, and project plans are suitable for implementation, that they meet the regulatory and customer requirements, and that they have continuity with existing processes and procedures.
The QC Scientist 1 creates procedures and forms for new methods, prepares periodic reports summarizing performance status of systems such as facility monitoring, method performance trending, Product Quality Reports, and other associated documentation. The individual contributes to or prepares protocols, summary reports, project plans, investigational reports, and other related quality system documentation. The position provides appropriate, innovative, and practical solutions to increasingly complex problems.
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Duties and Responsibilities:
- Performing a wide variety of procedures and techniques which may include, but are not limited to: cell culture, clinical/diagnostic microbiology, DNA analysis, DNA isolation, DNA purification, DNA sequencing, flow cytometry, gene cloning, manipulation of radioactive isotopes, natural product isolation, organic synthesis, peptide synthesis, protein analysis, RNA isolation, etc.
- Calibrating and operating a variety of laboratory instruments used to perform tests, analyses, and other laboratory procedures which may include, but are not limited to: flow cytometers, peptide synthesizers, ELISA readers, HPLC systems, spectrophotometers, microcentrifuges, autoclaves, etc.
- Generating laboratory records to support laboratory research, and independently performing necessary calculations (e.g. serial dilutions, molar concentrations etc.). Writing report(s) of findings, stating methods and procedures, including any modifications employed, specimens and materials involved, and results of experiments.
- Planning batch production record formulations in advance to coincide appropriately with other laboratory sections’ experimental needs.
- Interpreting, evaluating, and discussing the results of each experiment with the supervisor as part of the planning process for subsequent studies, and present these findings in individual weekly meetings with supervisor as well has lab group meetings.
- Preparing various buffers, stock supplies, and other reagents for use in the laboratory, cleaning and autoclaving glassware.
- Assisting in the analysis and interpretation of data and preparing summaries of laboratory findings to be used in reports and papers to be presented at scientific meetings and/or publication in scientific journals. Actively participating in the preparation of manuscripts, abstracts, and/or posters.
- Providing training to students and other laboratory personnel in the use of sophisticated laboratory equipment and in the performance of specialized procedures, techniques, etc.
- Responsible for laboratory upkeep and routine maintenance of equipment. Maintaining laboratory supplies and equipment including chemicals, biological materials, etc. required to handle biological materials, carcinogens, recombinant protein materials, acids, biological wastes, etc.
- Searching scientific literature for new methods and procedures that may be considered for application or modification to selected experiments; daily records of experiments and results in pen in bound laboratory notebooks; making minor modifications or adaptations to established methods, procedures, and/or techniques to solve problems in the work.
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PURPOSE, OBJECTIVES, FUNCTIONS:
- To conduct laboratory and pilot plant experiments to develop and improve bioreactor processes, media, cell lines, and operations.
- To develop a thorough understanding of the techniques and process and applies the technical knowledge to develop and implement a process, such that it is suitable for manufacturing.
- To execute internal and external client experimental plans in support of contracted work.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
- Design, supervise and evaluate laboratory experiments
- Develop economical and robust expression paradigms for recombinant proteins, through media development, cell line development and bioreactor process development
- Mentor junior staff members in laboratory techniques and procedures
- Provide initial interpretation of data and suggests next experimental steps
- Support process transfer into manufacturing and process validation at scale
- Identify process parameters for bioreactor and cell line development, and the necessary experimental analysis
- Maintain necessary documentation including process development reports, technical transfer reports, and process related documentation
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- Conducts research in liver and neuro toxicology using alternatives to classical, whole-animal test systems.
- Develops and/or identifies methods/biomarkers used for the screening of liver and neuro toxicity in dietary supplements and botanicals.
- Examines cell viability and appropriate functional biomarkers for each specified organ tissues, biological fluids and cells in culture for each organ tissue.
- Operates laboratory instrumentation such as spectrofluorometric and luminescence plate reader, flow cytometer (GUAVA), microfluidics, mass spectroscopy (LC/MS), and inverted and confocal microscopes.
- Develops all phases of research projects.
- Develops and implements use of biomarkers and other alternative safety assessment systems.
- Prepares written reports of scientific research data for public use.
Basic Requirement: Applicants must have a degree in physical sciences, life sciences, or engineering that included 30 semester hours in chemistry, supplemented by course work in mathematics through differential and integral calculus, and at least 6 semester hours of physics; OR a combination of education and experience — course work equivalent to a major as shown above, including at least 30 semester hours in chemistry, supplemented by mathematics through differential and integral calculus, and at least 6 semester hours of physics, plus appropriate experience or additional education.
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Reporting to the QM Process Chemistry Team Leader, the Process Chemist is responsible for providing support to process units with a chemistry-based knowledge of stream and additive characteristics to ensure the effective application of process controls. As part of the QM process chemistry team, the role is complemented and supported by the Quality Control and Quality Assurance functions. The ultimate objective is to monitor process activities and recommend initiatives to maintain or improve product quality to ensure that product released from manufacturing meets specifications and fit-for-purpose requirements.
- Manage (in conjunction with the Laboratory Supervisor) the balance of process samples required for laboratory testing versus field laboratories and on-line analyzers.
- Develop standards related to sample frequency, sampling location, testing turnaround and result reliability.
- Ensure the needs of operations data requirements are met in the most efficient and cost effective manner and interpreted to provide process units with timely and accurate data for decision-making.
- Monitor process quality performance through the establishment of Key Performance Indicators. Purpose is to evaluate the level of quality giveaway and risk in order to optimize the process financial contribution.
- Maintain expertise in the chemistry associated with assigned refinery process units.
- Provide consultation for process engineering and operations on process stream compositional chemistry issues and appropriate lab testing.
- Support operations with process additives and chemicals used for treatment to ensure appropriate chemistry is applied.
- Provide the lead in trouble-shooting for quality related operational upsets or where product fit-for-purpose is compromised.
- Maintain technical relationship with Shell Global Solutions Process Chemists for training and development.
- Lead process project initiatives that are related to product quality.
- Manage the effective application of on-line analyzers and field laboratories (selection, calibration and validation).
- Participate in development of Corrosion Control Documents and other Process Safety Initiatives that include refinery lab, field lab or on-line analyzer determination of critical process variables to control corrosion.
- Establish quality-training requirements for process operators and coordinate the development of training procedures (data interpretation, and impact of process deviations on product quality).
- Mentor Technical Associates and Chemist.
- Identify cost saving opportunities in the process operations.
- Maintain current technical knowledge in on-line equipment developments.
- Promote Quality Assurance systems to ensure effective application of quality processes and best practices.
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Scientist I – Chemist
San Diego, CA
The candidate will be responsible for the design and development of new therapeutic products for the Company, defining and applying novel methods.
- Generate and apply novel innovative ideas and approaches making major intellectual contributions to the success of the projects
- Design of experiments and evaluate biological testing data:
- Novel peptide formulation design and synthesis using “chemical concepts”
- Novel peptide design and synthesis
- Formulation development and improvement
- Scientific literature reading and search to maintain state of the art skills and knowledge
- Attend scientific meetings and conferences
- Recordkeeping in databases and ELN
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